Subunit Structure of l-Ribulokinase from Escherichia coli
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منابع مشابه
Crystalline L-ribulokinase from Escherichia coli.
r.-Ribulokinase has been purified and crystallized from induced cultures of Escherichia coli B/r mutant araA-2, a hyperproducer of this enzyme. The enzyme appears homogeneous by Sephadex G-200 column chromatography and sedimentation pattern. Recrystallization does not increase its specific activity. Acrylamide gel disc electrophoresis at pH 8.3 shows a trace of a slower component, not exceeding...
متن کاملReversions of the L-ribulokinase structural gene of Escherichia coli.
B C 28’ 22’ 15 I eu --79 6 16’ 74 70 26 43 23 63 71 60 14 62 80 55 46 25 I 29. 24 8 27 M U T A T I O N S in the araB gene, the structural gene for L-ribulokinase, of the arabinose gene-complex in Escherichia coli B/r (Figure 1) produce a twofold effect (ENGLESBERG 1961; LEE and ENGLESBERG 1962, 1963): they are deficient in L-ribulokinase, and they differ in their level of L-arabinose isomerase ...
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Asparaginase from Esckerickio coli B has been modified with increasing amounts of [r4C]succinic anhydride. Enzyme activity is enhanced when 15 % and 25 % of the lysyl residues are succinylated. Up to 40% of the lysyl residues were succinylated without destroying enzyme activity and without causing dissociation of this oligomeric protein. More extensive succinylation causes dissociation into sub...
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1. The molecular weights of the catalytic and regulatory polypeptide chains of aspartate transcarbamylase were determined to be 33,500 and 17,000, respectively. The results of three empirical methods are in good agreement with those obtained by sedimentation equilibrium in guanidine-hydrochloride or derived from the intrinsic viscosity and the sedimentation coefficient measured in the same solv...
متن کاملSuppression of a missense mutation in the L-ribulokinase gene of Escherichia coli.
HE L-arabinose gene-enzyme complex, except for the unlinked L-arabinose Tpermease gene, araE, is shown in Figure 1. araB and araA are the structural genes for the L-ribulokinase and L-arabinose isomerase, respectively; while araD is probably the structural gene for ~-ribulose-5-phosphate-4-epimerase ( ENGLESBERG 1961 ; ENGLESBERG et al. 1962; LEE and ENGLESBERG 1962,1963). The ma€ gene is a reg...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)63244-2